Transporter Products
Transporter Catalog
GenoMembrane membranes and vesicles are prepared by technically advanced methodologies, using plasma membrane purified from an insect cell system (Sf9 cells transfected with baculovirus) over-expressing ABC transporters. These products are very effective for the in vitro screening of transporter substrates and inhibitors in a 96-well plate format.
The ATP-binding cassette (ABC) transporters are a family of membrane proteins that exist in various organs, including the intestine, liver, kidney, and blood brain barrier. These molecules have ATPase activity and mediate ATP-dependent transport of various drugs, fats, sterols, and endogenous metabolites. Forty eight ABC transporters have so far been identified, and approximately eleven are known to transport compounds across cell membranes. Evaluating the interaction of drug candidates with these transporters early in the drug development process can help with the selection of drugs with high efficacy and low potential for adverse effects.
ABC Transporter Membranes may be utilized in an ATPase assay to determine possible interactions between transporter and test compound. Driven by the hydrolysis of ATP, ABC transporters mediate drug efflux from cells, making it is important to know if a compound is a substrate or inhibitor of ABC transporters when predicting the ADME properties of a candidate drug.
ABC Transporter Vesicles, which have an inside-out structure, are prepared from ABC transporter membranes for use
in vesicular transport assays. While
ABC transporters typically mediate the efflux of subtrates from cells, transporters expressed on these inside-out vesicles import
substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for a drug candidate by
determining of the amount of compound incorporated into the vesicles. Vesicular transport assays using ABC Transporter Vesicles
are simple, effective tools for in vitro screening of transporter substrates and inhibitors.
