Cryopreserved Hepatocytes
Human Inventory
Human Cryopreserved For Suspension Use - Metabolism Applications
Human Cryopreserved For Enzyme Induction Applications
Human Cryopreserved For Culture Use – In Biliary Excretion
Human Cryopreserved For Culture Use – In Plated Metabolism
Animal Inventory
Animal Cryopreserved Characterization/Inventory
Example of a Product Characterization Sheet
PCS-Lot# Hu0910
PCS-Lot# Hu0939
PCS-Lot# Hu8064
PCS-Lot# Hu8072
PCS-Lot# HuP58
CellzDirect has built an extensive inventory of superior grade animal and human cryopreserved hepatocytes. We have carefully produced and characterized our lots, offering detailed donor demographics, extensive P450 profiling, responsiveness to inducers, morphological assessment, and application suitability. We offer pooled and single donor human and animal lots, including an ample supply of plateable lots from all standard toxicological species that are suitable for a variety of applications. For your convenience we offer application-specific kits for thawing and plating. Please review our current animal and human inventory lists and characterization data. Experienced scientists in our Technical Support & Customer Service Department are standing by to assist you:
1-866-952-3559 or +1-919-545-9959 hepaticproducts@invitrogen.com
Cryopreserved hepatocytes have more recently become mainstays for in vitro researchers worldwide. Responding to this demand, CellzDirect scientists, partnered with APS, Inc., have worked painstakingly to develop a large inventory of high quality human cryopreserved cells. We offer five basic application-based categories of cryopreserved hepatocytes: Hepatocytes For Suspension Use - Metabolism Applications, Pooled Human Cryopreserved For Suspension Use - Metabolism Applications, Hepatocytes For Culture Use - Metabolism Applications, Hepatocytes For Enzyme Induction Applications and Human Cryopreserved For Biliary Excretion Applications. For your convenience, we offer a Cryopreserved Hepatocyte Evaluation Kit (CHEK) and application medium.
Cryopreserved Hepatocyte Standard Species Available
Human, Non-human primate (Baboon, Cynomolgus, Rhesus), Dog (Beagle), Rabbit (New Zealand White), Rat (Sprague-Dawley, Wistar), Mouse (CD-1)
Custom Species Available Upon Request
Lot Characterization
Due to the wide variety of applications cryopreserved hepatocytes are used for, CellzDirect has worked to provide the most thorough lot characterization data in the industry. Extensive characterization information is provided with each lot that you purchase and minimally includes:
*Post Thaw Cell Viability and Yield
*Incubation Stability
*Phase I & II Enzyme Activities
*Morphological Assessment with Representative Images
Our human plateable lots undergo additional evaluation which includes a suitability assessment for common application such as: metabolic stability, enzyme induction, and biliary excretion. To provide you with the best plating conditions for your specific application, we offer optimized plating and culture conditions. As part of this evaluation, we include a multi-day quality and functional assessment of the monolayer and complete characterization of the induction profile for each lot.
*Donor Demographics
*Optimized Seeding Density
*Attachment Efficiency
*Morphological Assessment of the Monolayer with Representative Images
*Suitability for Drug Transport Studies
*Cytochrome P450 Induction (CYP1A2, CYP2B6, and CYP3A4)
Formats and Shipping
CellzDirect’s cryopreserved hepatocytes are from male animals unless otherwise requested. The human hepatocytes are from both male and female, depending on availability. Cryopreserved hepatocytes are shipped Monday through Thursday by FedEx Priority. Orders received after 12 P.M. EST are shipped the following business day. Contact us if you require special shipping arrangements.
For research purposes only; not to be used for clinical application or redistribution. CellzDirect cell products may contain human or other primate source material that should be treated as potentially hazardous. Use universal precautions for handling biohazardous material.
